Characterization of the palytoxin effect on Ca2+-ATPase from sarcoplasmic reticulurn (SERCA)

The effect of palytoxin was studied in a microsomal fraction enriched in longitudinal tubules of the Sarcoplasmic reticulum membrane. Half-maximal effect of palytoxin on Ca2+-ATPase activity yielded an apparent inhibition constant of approx. 0.4 [mu M. The inhibition process exhibited the following chatacteristics: (i) the degree of inhibition was dependent on membrane protein concentration; (ii) no protection was observed when the ATP concentration was raised; (iii) dependence on Ca2+ concentration with a decreased maximum catalytic rate; (iv) it Occurred in the absence Or Ca2+ ionophoric activity. Likewise, the inhibition mechanism was linked to: (i) rapid enzyme phosphorylation from ATP in the presence of Ca2+ but lower steady-state levels of phosphoenzyme; (ii) more drastic effect on phosphoenzyme levels when the toxin was added to the enzyme in the absence of Ca2+; (iii) decreased phosphoenzyme levels at saturating Ca2+ concentrations: (iv) no effect on kinetics of phosphoenzyme decomposition. The palytoxin effect is related with lock of the enzyme in the Ca2+-free conformation so that progression of the catalytic cycle is impeded. (c) 2008 Elsevier Inc. All rights reserved.