Journal
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
Volume 91, Issue 3, Pages 297-304Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijfoodmicro.2003.07.004
Keywords
Listeria monocytogenes; PCR; transcriptional regulator; virulence
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Listeria monocytogenes is an opportunistic bacterial pathogen that has accounted for an important portion of human foodbome diseases worldwide. In this study, through comparative analysis of L. innoctia and L. monocytogenes genomic sequences. we selected a L. monocytogenes specific gene (lmo0733) that has the potential for specific detection of L. monocytogenes. Using PCR primers (lmo0733F and lmo0733R) derived from this gene, a specific fragment of 453 bp was amplified only from genomic DNA of L. monocytogenes strains. PCR products from other Listeria species as well as other Gram-positive and -negative species were not detectable, confirming the specificity of this assay. Thus, the PCR test employing primers lmo0733F and lmo0733R represents an additional tool in the diagnostic arsenal for rapid, sensitive and specific detection and identification of human infections due to L. monocytogenes. (C) 2003 Elsevier B.V. All rights reserved.
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