Journal
ANALYTICAL BIOCHEMISTRY
Volume 326, Issue 2, Pages 153-166Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2003.11.025
Keywords
laser tweezers; back focal-plane interferometry; single-molecule studies; kinesin; multitasking
Funding
- NHLBI NIH HHS [HL64381] Funding Source: Medline
- NIAMS NIH HHS [AR45604] Funding Source: Medline
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We present a design for implementing multiple laser traps for single-molecule studies through time-sharing using commercially available digital signal processing hardware in a computer running a standard multitasking operating system. This design enables four to six independent laser traps with a visitation frequency of 10,000s(-1) trap(-1) and a timing jitter of +/-0.5 mus to be created. The design also achieves nanometer-resolution detection of displacement in all of the traps simultaneously via back focal-plane interferometry and only a single quadrant photodiode detector. Practical design considerations and limitations together with the use of fiberlasers in laser traps are discussed. Using this device, the mechanokinetics of multiple molecular motors or adhesion proteins may be measured simultaneously. We present the example biological application of two kinesin-coated beads in separate traps moving on different portions of a microtubule. (C) 2004 Elsevier Inc. All rights reserved.
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