Journal
BIOSENSORS & BIOELECTRONICS
Volume 19, Issue 8, Pages 875-883Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2003.08.014
Keywords
nanoparticles; DNA microarray; silver amplification; SNPs; Staphylococcus; thrombosis
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Funding
- NHLBI NIH HHS [1 R43 HL65876-01] Funding Source: Medline
- NIGMS NIH HHS [1 R43 GM62096-01A1, R43 GM062096-01A1] Funding Source: Medline
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The development of a nanoparticle-based detection methodology for sensitive and specific DNA-based diagnostic applications is described. The technology utilizes gold nanoparticles derivatized with thiol modified oligonucleotides that are designed to bind complementary DNA targets. A glass surface with arrays of immobilized oligonucleotide capture sequences is used to capture DNA targets, which are then detected via hybridization to the gold nanoparticle probes. Amplification with silver allows for detection and quantitation by measuring evanescent wave induced light scatter with low-cost optical detection systems. Compared to Cy3-based fluorescence, silver amplified gold nanoparticle probes provide for a similar to1000-fold increase in sensitivity. Furthermore, direct detection of non-amplified genomic DNA from infectious agents is afforded through increased specificity and even identification of single nucleotide polymorphisms (SNP) in human genomic DNA appears feasible. (C) 2003 Elsevier B.V. All rights reserved.
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