Journal
CANCER LETTERS
Volume 205, Issue 2, Pages 181-188Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2003.11.027
Keywords
gene methylation; prostate cancer; early detection; prognosis
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Gene methylation is an important molecular event in prostate carcinogenesis that may have diagnostic and prognostic significance. We evaluated the methylation status of eight genes implicated in prostate carcinogenesis. DNA was extracted from archived paraffin-embedded tumor blocks from 90 prostate cancer patients. Gene methylation status of eight genes (GSTP1, RASSF1A, RARP2, CD44, EDNRB, E-cadherin, Annexin-2, and Caveolin-1) was determined using real-time. methylation-sensitive PCR techniques. Differences in gene methylation among race, tumor grade and disease stage were evaluated by chisquare test. Of the eight genes, GSTP1, RASSF1A, and RARP2 methylation was highly prevalent across tumors (> 60% for all three genes) whereas CD44, E-cadherin and EDNRB methylation was less prevalent (33, 24 and 29%, respectively). Annexin-2 and Caveolin-1 were not methylated in any of the tumors examined. Methylation of RARP2, CD44 and E-cadherin was correlated with tumor grade but not stage. Interestingly, methylation of EDNRB, a gene involved in angiogenesis, was correlated with stage of disease but not tumor grade. With the possible exception of CD44, we did not observe differences in gene methylation between racial categories for the genes under study. In summary, our data suggest that evaluation of the methylation of a panel of genes may have diagnostic and prognostic utility in prostate cancer. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
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