Journal
CHEMPHYSCHEM
Volume 5, Issue 3, Pages 367-372Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cphc.200300978
Keywords
bioinformatics; microarrays; nanostructures; nucleic acids; self-assembly
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We report on the microarray-based in vitro evaluation of two libraries of DNA oligonucleotide sequences, designed in silico for applications in supramolecular self-assembly, such as DNA computing and DNA-based nanosciences. In this first study which is devoted to the comparison of sequence motif properties theoretically predicted with their performance in real-life, the DNA directed immobilization (DDI) of proteins was used as an example of DNA-based self-assembly. Since DDI technologies, DNA computing, and DNA nanoconstruction essentially depend on similar prerequisites, in particular, large and uniform hybridization efficiencies combined with low nonspecific cross-reactivity between individual sequences, we anticipate that the microarray approach demonstrated here will enable rapid evaluation of other DNA sequence libraries.
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