Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1030, Issue 1-2, Pages 43-51Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2003.12.027
Keywords
optimization; mobile phase composition; preparative chromatography; pharmaceutical analysis; drugs; ammonium hydrogencarbonate
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Careful selection of both high-pH mobile phase as well as organic modifier, was performed in order to develop and optimize HPLC conditions for the separation of drug discovery compounds. High-pH mobile phases provide excellent chromatographic resolution and increased mass loading of basic compounds. The analytical methods so defined have been successfully transferred to preparative automated UV-directed purification, an important fact due to the increasing number of samples requiring purification. It should be noted that, the single prerequisite for this approach is an analytical LC-UV-MS run, therefore the system has the ability to collect only fractions likely to contain the target product. A cost-effective strategy for maximizing the purification of drug discovery compounds is proposed. (C) 2004 Elsevier B.V. All rights reserved.
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