Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 85, Issue 6, Pages 666-675Publisher
WILEY
DOI: 10.1002/bit.10880
Keywords
Plackett-Burman; medium limitations; factor screening
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Here we report the successful implementation of the Plackett-Burman multifactorial design to screen the limiting components for growth and subsequent use of the response surface methodology (RSM) to design a medium that supported exponential growth of the aggregated morphology of the shipworm bacterium, Teredinobacter turnirae. The results obtained with the help of Plackett-Burman design indicated limitations of three components in the growth medium, MnCl(2)(.)4H(2)O, Na2CO3, and K2HPO4. The concentrations of these three components were further optimized using RSM. By increasing the concentrations of the above-mentioned components by 4-fold, 12-fold, and 12-fold, respectively, it became possible to achieve exponential growth of the culture. (C) 2004 Wiley Periodicals, Inc.
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