Journal
VIROLOGY
Volume 321, Issue 1, Pages 47-56Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2003.10.028
Keywords
positive-strand RNA virus; replication; translation; minus strand synthesis; RNA-dependent RNA polymerase; turnip yellow mosaic virus; tRNA-like structure
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Funding
- NIGMS NIH HHS [GM54610] Funding Source: Medline
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The genomic RNA of Turnip yellow mosaic virus (TYMV) has an 82-nucleotide-long tRNA-like structure at its 3'-end that can be valylated and then form a stable complex with translation elongation factor eEF1A(.)GTP. Transcription of this RNA by TYMV RNA-dependent RNA polymerase (RdRp) to yield minus strands has previously been shown to initiate within the 3'-CCA sequence. We have now demonstrated that minus strand synthesis is strongly repressed upon the binding of eEF1A(.)GTP to the valylated viral RNA. eEF1A(.)GTP had no effect on RNA synthesis templated by non-aminoacylated RNA. Higher eEF1A(.)GTP levels were needed to repress minus strand synthesis templated by valyl-EMV TLS RNA, which binds eEF1A(.)GTP with lower affinity than does valyl-TYMV RNA. Repression by eEF1A(.)GTP was also observed with a methionylated variant of TYMV RNA and with aminoacylated tRNA(His), tRNA(Ala), and tRNA(Phe) transcripts. It is proposed that minus strand repression by eEF1A(.)GTP binding occurs early during infection to help coordinate the competing translation and replication functions of the genomic RNA. (C) 2004 Published by Elsevier Inc.
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