Journal
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
Volume 58, Issue 3, Pages 187-193Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbbm.2003.10.008
Keywords
lipid extraction; proteins; organic solvents; silica; detergent
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The present work compares six biochemical methods for extraction of lipids from human serum. Although some organic solvents were good lipid extractors, they precipitated most of the total proteins and albumin. On the other hand, methodologies using Triton X-114 and silica were efficient for extraction of lipids, while sparing the protein fraction. (C) 2003 Elsevier B.V. All rights reserved.
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