Journal
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Volume 424, Issue 1, Pages 89-96Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2004.01.016
Keywords
D-aspartate; rat pheochromocytoma cells; L-glutamate transporter; DL-TBOA
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In previous studies [FEBS Lett. 434 (1998) 231, Arch. Biochem. Biophys. 404 (2002) 92], we demonstrated for the first time that D-aspartate (D-Asp) is synthesized in cultured mammalian cell lines, such as pheochromocytorna 12 (PC12) and its subclone, MPTI. Our current focus is analysis of the dynamics of D-Asp homeostasis in these cells. In this communication, we show that L-glutamate (Glu) and L-Glu transporter substrates in the extracellular space regulate the homeostasis of endogenous D-Asp in MPT1 Cells. D-Asp is apparently in dynamic homeostasis, whereby endogenOUS D-Asp is constantly released into the extracellular space by an undefined mechanism, and continuously and intensively taken up into cells by an L-Glu transporter. Under these conditions, L-Glu and its transporter substrates in the medium may competitively inhibit the uptake Of D-Asp via the transporter, resulting in accumulation of the amino acid in the extracellular space. We additionally demonstrate that DL-TBOA, a well-established L-Glu transporter inhibitor, is taken up by the transporter during long time intervals, but not on a short time-scale. (C) 2004 Elsevier Inc. All rights reserved.
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