Journal
BIOTECHNOLOGY LETTERS
Volume 26, Issue 8, Pages 671-674Publisher
SPRINGER
DOI: 10.1023/B:BILE.0000023028.21985.07
Keywords
alkaloids; Catharanthus roseus; shoot cultures; vindoline
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Vindoline, the major alkaloid in cultures of Catharanthus roseus shoots, reached 2 mg g(-1) dry wt after 27 d in culture. Maximal vindoline accumulation coincided with maximum activities of deacetoxyvindoline 4-hydroxylase, deacetylvindoline acetyl-CoA acetyl transferase and tryptophan decarboxylase. Shoot exposure to jasmonate shortened the time required for the maximal vindoline accumulation to 14 d.
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