Journal
STRUCTURE
Volume 12, Issue 4, Pages 611-622Publisher
CELL PRESS
DOI: 10.1016/j.str.2004.02.019
Keywords
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Funding
- MRC [MC_U117584256] Funding Source: UKRI
- Medical Research Council [MC_U117584256] Funding Source: Medline
- Telethon [1278] Funding Source: Medline
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Z band alternately spliced PDZ-containing protein (ZASP) is a sarcomere Z disk protein expressed in human cardiac and skeletal muscle that is thought to be involved in a dominant familial dilated cardiomyopathy. The N-terminal PDZ domain of ZASP interacts with the C terminus of alpha-actinin-2, the major component of the Z disk, probably by forming a ternary complex with titin Z repeats. We have determined the structure of ZASP PDZ by NMR and showed that it is a classical class 1 PDZ domain that recognizes the carboxy-terminal sequence of an a-actinin-2 calmodulin-like domain with micromolar affinity. We also characterized the role of each component in the ternary complex ZASP/alpha-actinin-2/titin, showing that the alpha-actinin-2/ZASP PDZ interaction involves a binding surface distinct from that recognized by the titin Z repeats. ZASP PDZ structure was used to model other members of the enigma family by homology and to predict their abilities to bind a-actinin-2.
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