Journal
PURE AND APPLIED CHEMISTRY
Volume 76, Issue 4, Pages 829-837Publisher
WALTER DE GRUYTER GMBH
DOI: 10.1351/pac200476040829
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Proteomics studies rely on high-resolving separation techniques whereby the biological complexity can be unraveled. Both gel-based and liquid-phase separation principles are needed in order to mine deeper into the low abundant area of the proteins expressed in human cell samples. The expression levels that can be reached today with proteomics technology are still only reaching part of all proteins present in complex biological samples. The challenge to be met is not an easy task and will, in many aspects, be determined by the speed and success whereby the analytical field can make milestone progress. Examples will be given from our research group on proteomics studies, as well as principles and challenges within this research area.
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