Journal
THIN SOLID FILMS
Volume 453, Issue -, Pages 383-387Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.tsf.2003.11.161
Keywords
MAPLE DW; tissue engineering
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A laser-based transfer technique, termed matrix assisted pulsed laser evaporation direct write (MAPLE DW), has been modified to rapidly and accurately deposit mammalian cells in a non-contact manner. While this technology was originally developed for deposition of inorganic materials, it has shown the ability to transfer of a wide range of biological materials. Two types of mammalian cells, human osteosarcoma and rat cardiac cells were deposited into a biopolymer matrix via MAPLE DW Current results show that it is possible to deposit cells in a stepwise manner and build cellular 'stacks' 50-100 mum tall. Furthermore, the technique is now capable of depositing cells with near single cell resolution. Post-transfer results of live/dead viability/cytotoxicity assays show that the cells are unaffected by the process with near 100% viability. The ability to build cellular structures in three dimensions and deposit small numbers of cells accurately has potential application to tissue engineering. (C) 2003 Elsevier B.V. All rights reserved.
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