Journal
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
Volume 286, Issue 4, Pages F653-F659Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajprenal.00163.2003
Keywords
glomerular mesangial cells; Janus kinase/signal transducers and activators of transcription pathway
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Funding
- NHLBI NIH HHS [HL 58139, HL-60653] Funding Source: Medline
- NIDDK NIH HHS [DK-50268] Funding Source: Medline
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Clinical and animal studies show that treatment with angiotensin-converting enzyme ( ACE) inhibitors or ANG II-receptor antagonists slows progression of nephropathy in diabetes, indicating ANG II plays an important role in its development. We previously reported that hyperglycemia augments both ANG II-induced growth and activation of Janus kinase (JAK) 2 and signal transducers and activators of transcription ( STAT) proteins in cultured rat mesangial cells. Furthermore, we demonstrated that the tyrosine kinase enzyme JAK2 plays a key role in both ANG II- and hyperglycemia-induced growth in these cells. We hypothesized that the ACE inhibitor captopril and the ANG II- receptor antagonist candesartan would hinder hyperglycemic-induced activation of JAK and STAT proteins in rat glomeruli, demonstrating that ANG II plays an important role in the activation of these proteins in vivo. Adult male Sprague-Dawley rats were given either streptozotocin ( STZ; 60 mg/kg iv) or vehicle, and glomeruli were isolated 2 wk later. Activation of JAK and STAT proteins was evaluated by Western blot analysis for specific tyrosine phosphorylation. Groups of rats were given captopril (75 - 85 mg . kg(-1) . day(-1)), candesartan (10 mg . kg(-1) . day(-1)), or the JAK2 inhibitor AG-490 (5 mg . kg(-1) . day(-1)) for the study's duration. STZ stimulated glomerular phosphorylation of JAK2, STAT1, STAT3, and STAT5. Phosphorylation was reduced in rats treated with captopril, candesartan, and AG-490. Furthermore, both candesartan and AG-490 inhibited STZ- induced increases in urinary protein excretion. In conclusion, our studies demonstrate that hyperglycemia induces activation of JAK2 and the STATs in vivo via an ANG II- dependent mechanism and that these proteins may be involved in the early kidney damage associated with diabetes.
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