Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 68, Issue 4, Pages 939-941Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.68.939
Keywords
bioconversion; N-methyltransferase; tetrahydroisoquinoline
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Coclaurine N-methyltransferase from Coptis japonica catalyzes the N-methylation of coclaurine as well as simple tetrahydroisoquinoline. We examined the possibility of converting 6,7-dimethoxy-1,2,3,4-tetrahydro-isoquinoline into its N-methylated product using trans-genic Escherichia coli, which expressed recombinant coclaurine N-methyltransferase, without the addition of a methyl-group donor. Transgenic E. coli successfully N-methylated the substrate added to the medium and excreted the product. Limitation of bioconversion by the supply of methyl-group donor is discussed.
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