Journal
MOLECULAR PLANT-MICROBE INTERACTIONS
Volume 17, Issue 4, Pages 383-393Publisher
AMER PHYTOPATHOLOGICAL SOC
DOI: 10.1094/MPMI.2004.17.4.383
Keywords
fungal transcription factor; host-pathogen interaction
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CPTF1, a transcription factor with significant homology to ATF/CREB bZIP factors, was identified during an expressed sequence tag (EST) analysis of in planta-expressed genes of the phytopathogen Claviceps purpurea. Using a gene-replacement approach, deletion mutants of cptf1 were created. Expression studies in axenic culture showed that the H2O2-inducible gene cpcat1 (encoding a secreted catalase) had a reduced basal expression level and no longer responded to oxidative stress in the Acptf1 mutant. Biochemical analyses indicated that CPTF1 is a general regulator of catalase activity. Acptf1 mutants showed significantly reduced virulence on rye. Electron microscopical in situ localization revealed significant amounts of H2O2 in Deltacptp1-infected rye epidermal tissues, indicating that the plant tissue displayed an oxidative burst-like reaction, an event not detected in wild-type infections. These data indicate that CPTF1 is involved not only in oxidative stress response in the fungus but also in modulation of the plant's defense reactions.
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