Journal
JOURNAL OF BACTERIOLOGY
Volume 186, Issue 8, Pages 2495-2498Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.186.8.2495-2498.2004
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Funding
- NIAID NIH HHS [AI12202, R01 AI012202] Funding Source: Medline
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Salmonella FliR and FlhB are membrane proteins necessary for flagellar export. In Clostridium afliR-flhB fusion gene exists. We constructed a similar Salmonella fusion gene which is able to complement fliR,flhB, and fliR flhB null strains. Western blotting revealed that the FliR-FlhB fusion protein retains the FlhB protein's cleavage properties. We conclude that the FliR and FlhB proteins are physically associated in the wild-type Salmonella basal body, probably in a 1:1 ratio.
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