Journal
DIGESTIVE DISEASES AND SCIENCES
Volume 49, Issue 4, Pages 556-564Publisher
SPRINGER
DOI: 10.1023/B:DDAS.0000026298.72088.f7
Keywords
dextran sulfate sodium; inflammatory bowel disease; disease activity index; myeloperoxidase assay; histology
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Funding
- NIDDK NIH HHS [P01 DK43785] Funding Source: Medline
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In dextran sulfate sodium (DSS)-induced inflammatory bowel disease in mice the relationship between the amount of ingested DSS and the severity of colitis has not been systematically investigated. We examined whether (1) the severity of colitis is DSS load-dependent, and (2) there is a critical DSS load required to reliably induce colitis. DSS load was calculated as: (drinking volume (ml) x [DSS (g)/100 ml])/body weight (g). A minimum DSS load greater than or equal to30 mg/g body weight over 7 days resulted in a significantly elevated colonic myeloperoxidase (MPO) activity, compared to mice receiving less DSS and controls (P < 0.05). Histomorphologic data correlated with MPO activity and revealed significantly higher damage scores once the DSS load was >= 30 mg/g body weight. Our findings demonstrate the importance of monitoring DSS load in this model of experimental colitis.
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