Journal
CLINICAL MICROBIOLOGY AND INFECTION
Volume 10, Issue 4, Pages 332-334Publisher
ELSEVIER SCI LTD
DOI: 10.1111/j.1198-743X.2004.00818.x
Keywords
Mycobacterium; tuberculosis; diagnosis; IS6110; 16S rDNA; strand displacement amplification
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The BDProbeTec MTB assay for direct detection of Mycobacterium tuberculosis was evaluated in comparison with the AMTD-II assay on 94 samples from different patients with clinical suspicion of tuberculosis. Using a combination of culture on Lowenstein-Jensen medium (with or without preculture in BACTEC 9000) and clinical diagnosis as the standard, BDProbeTec MTB showed high sensitivity and specificity (96.1% and 100%, respectively), similar to AMTD-II (96.1% and 97.1%, respectively), with significantly higher sensitivity than the Ziehl-Neelsen stain for acid-fast bacilli (73%, p < 0.05).
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