Journal
WEED TECHNOLOGY
Volume 18, Issue 2, Pages 464-472Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1614/WT-03-047R1
Keywords
least significant difference; linear mixed models; repeated measures; tank mixture
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When herbicides are applied in mixture, and infestation by weeds is less than expected compared with when herbicides are applied alone, a synergistic effect is said to exist. The inverse response is described as being antagonistic. However, if the expected response is defined as a multiplicative, nonlinear function of the means for the herbicides when applied alone, then standard linear model methodology for tests of hypotheses does not apply directly. Consequently, nonlinear mixed-model methodology was explored using the nonlinear mixed-model procedure (PROC NLMIXED) of SAS System(R). Generality of the methodology is illustrated using data from a randomized block design with repeated measures in time. Nonlinear mixed-model estimates and tests of synergistic and antagonistic effects were more sensitive in detecting significance, and PROC NLMIXED was a versatile tool for implementation.
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