Journal
MITOCHONDRION
Volume 3, Issue 5, Pages 251-259Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.mito.2004.02.001
Keywords
human Tomm70 gene; NRF-2 transcription factor; mitochondrial protein transport
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TOMM70 is a subunit of the outer mitochondrial membrane translocase that plays a major role as a receptor of hydrophobic pre-proteins targeted to the mitochondria. We report the presence of two nuclear respiratory factor 2 (NRF-2) binding motifs in the 5'-flanking region of the human Tomm70 gene and establish their essential role for promoter activity by using reporter assays in HeLa cells. We show that both NRF-2 binding sites are functional and present evidence that interactions between these sites and a CpG island contribute to expression. Mobility shift assays show that these NRF-2 sites are specifically recognized by NRF-2 present in HeLa nuclear extracts. (C) 2004 Elsevier B.V. and Mitochondria Research Society. All rights reserved.
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