Journal
ELECTROPHORESIS
Volume 25, Issue 7-8, Pages 1120-1124Publisher
WILEY
DOI: 10.1002/elps.200305784
Keywords
capillary electrophoresis; electroosmotic flow; microfluidics; poly(dimethylsiloxane)
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Poly(dimethylsiloxane) (PDMS) microfluidic devices were prepared from different ratios of curing agent (which contains silicon hydride groups) to base (which contains vinyl-terminated noncross-linked PDMS), to determine the effect of this ratio on electroosmotic flow (EOF). In fabricating devices for this purpose, a novel method for permanently enclosing PDMS channels was developed. As a supplement to the microfluidic method, the inner walls of capillaries were coated with PDMS formed from varying ratios of curing agent to base. EOF was found to be constant for PDMS formed with each ratio, which implies that the negative surface charges do not arise from chemical species present only in the base or the curing agent.
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