Journal
ANALYTICA CHIMICA ACTA
Volume 507, Issue 1, Pages 129-135Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2003.12.039
Keywords
microfluidic chips; sequential injection; solid-phase reactors; chemiluminescence; immobilized enzymes; glucose
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A three-layer poly(dimethylsiloxane) (PDMS)/glass microfluidic system for performing on-chip solid-phase enzymatic reaction and chemiluminescence (CL) reaction was used for the determination of glucose as a model analyte. A novel method for the immobilization of controlled-pore-glass based reactive particles on PDMS microreactor beds was developed, producing an on-chip solid-phase reactor that featured large reactive surface and low flow impedance. Efficient mixing of reagent/sample/carrier streams was achieved by incorporating chaotic mixer structures in the microfluidic channels. A conventional sequential injection (SI) system was adapted for direct coupling with the microfluidic system, and combined with hydrostatic delivery of reagents to achieve efficient and reproducible sample introduction at 10 mu1 levels. A detection limit of 10 muM glucose (3sigma), and a precision of 3.1% RSD (n = 7, 0.2 mM glucose) were obtained using the SI-microfluidic-CL system integrated with a glucose oxidase (GOD) reactor. Carryover was < 5% at a throughput of 20 samples/h. (C) 2003 Elsevier B.V. All rights reserved.
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