Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1032, Issue 1-2, Pages 203-211Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2003.10.038
Keywords
field-enhanced sample stacking; peptides; proteins
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Field-enhanced sample injection (FESI) was used to improve the concentration sensitivity of a capillary electrophoresis (CE)-mass spectrometry (MS) system with sheath flow configuration. Using some bioactive peptides, more than 3000-fold improvement in signal was obtained, permitting analysis in the low nM (fmol/mul) levels. The system was further evaluated for analysis of complex peptide mixtures by using low concentration tryptic digests of standard proteins. Rapid identification of the original protein was obtained by database searching using the observed molecular masses of the peptides, and by comparison of actual MS-MS spectra of selected peptides with the predicted fragmentation patterns. (C) 2003 Elsevier B.V. All rights reserved.
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