Journal
BIOPOLYMERS
Volume 73, Issue 5, Pages 597-605Publisher
WILEY
DOI: 10.1002/bip.20006
Keywords
covalent attachment; immobilization; DNA : glass beads; hybridization
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Several covalent attachment chemistries were tested for the immobilization of DNA onto glass beads. The comparison was based on the ability of these chemistries to produce derivatized beads that give good hybridization signals. Cyanuric chloride, isothiocyanate, nitrophenyl chloro,formate, and hydrazone chemistries gave its the best (yet comparable) hybridization signals. We further characterized the cyanuric chloride method for the number of attachment sites, number of hybridizable sites, hybridization kinetics, effect of linker length on hybridization intensity and stability of the derivatized beads. (C) 2004 Wiley Periodicals, Inc.
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