Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 802, Issue 2, Pages 329-338Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2003.12.015
Keywords
prednisone; prednisolone; dexamethasone; cortisol
Funding
- NCRR NIH HHS [S10RR14572] Funding Source: Medline
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Glucocorticoids are an important component of immunosuppressive therapy for solid organ transplantation. A method to quantitate prednisone, prednisolone, dexamethasone and cortisol in human serum has been developed. Analysis is performed utilizing reversed-phase liquid chromatography coupled to tandem mass spectrometry. The method was validated to a lower limit of quantitation of 5.4 ng/ml for prednisone and cortisol, and 10.7 ng/ml for dexamethasone and prednisolone, with error below 7% at the lower limits. The between-day relative standard deviations ranged 2.9-7.1%. Comparison of cortisol analysis to an established method using clinical samples yielded differences below 15% for 26 of 28 determinations. (C) 2004 Elsevier B.V. All rights reserved.
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