Journal
FEBS LETTERS
Volume 563, Issue 1-3, Pages 108-112Publisher
WILEY
DOI: 10.1016/S0014-5793(04)00279-0
Keywords
ion specificity; osmosensor; osmoregulator; potassium; sodium; Corynebacterium glutamicum
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The Na+/betaine carrier BetP from Corynebacterium glutamicum was purified and reconstituted in Escherichia coli phospholipid liposomes and its osmosensory properties were studied with respect to the cation specificity of osmotic activation. To dissect the influence of the co-substrate Na+ on the energetics of uptake from its possible role as a putative trigger of osmolality-dependent BetP activation, the internal Na+ concentration was varied without changing ApNa(+). Studying betaine uptake at increasing luminal Na+ or K+ revealed that BetP activity was triggered by Na+ only to a negligible extent compared to activation by K+. We conclude that activation of BetP in proteoliposomes depends solely on K+, both in mechanistic and in physiological terms. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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