Journal
BIOSENSORS & BIOELECTRONICS
Volume 19, Issue 9, Pages 1121-1124Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2003.06.001
Keywords
nitric oxide; endothelial cell; cellular biosensing system; acetylcholine NOC 7; L-NMMA; blood pressure
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In order to assess blood pressure control drugs, the endothelial cellular biosensing system for assessing blood pressure control drugs was constructed. This system consists of human umbilical vein endothelial cells (HUVEC) on a polyion-coated gold electrode, a platinum counter electrode and an Ag/AgCl reference electrode. Nitric oxide (NO) as an indicator of blood vessel relaxation was detected with a polyion-coated electrode in the system. The NO detection limit of this electrode was 8.4 nM by differential pulse voltammetry (DPV). The drugs of blood pressure control (acetylcholine chloride (AcChCl), NOC 7 and N-G-monomethyl-L-arginine (L-NMMA)) were assessed with this endothelial cellular biosensing system. One milli molar of AcChCl make NO released from HUVEC stimulated by activating endothelial nitric oxide synthase (eNOS) in HUVEC. In the case of 5 mM Of L-NMMA, NO releasing was inhibited by inhibiting eNOS activation by I mM of AcChCl. NOC 7 immediately released NO regardless of eNOS activation in endothelial cells. (C) 2003 Elsevier B.V. All rights reserved.
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