Journal
MOLECULAR AND CELLULAR ENDOCRINOLOGY
Volume 218, Issue 1-2, Pages 107-118Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2003.12.007
Keywords
estrogen receptor; vitellogenin; real-time PCR; reproduction; largemouth bass
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Funding
- NIEHS NIH HHS [P42 ES 07375] Funding Source: Medline
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The expression levels of three estrogen receptor (ER) isotypes alpha, beta, and gamma were quantified in female largemouth bass (Micropterus salmoides) (LMB) liver, ovary, brain, and pituitary tissues. ER alpha and beta expression predominated in the liver, while ERs beta and gamma predominated in the other tissues. Temporally in females, ER alpha was highly up-regulated, ER gamma was slightly up-regulated, and ER beta levels remained unchanged in the liver when plasma 17-beta estradiol (E-2) and vitellogenin (Vtg) levels were elevated in the spring. In ovarian tissue from these same fish, all three ERs were maximally expressed in the fall, during early oocyte development and prior to peak plasma E-2 levels. When males were injected with E-2, ER alpha was highly inducible, ER gamma was moderately up-regulated, and ER beta levels were not affected. None of the ER isotypes were induced by E-2 in gonadal tissues. These results combined suggest that the ERs themselves are not regulated in the same manner by E-2, and furthermore, do not contribute equally to the transcriptional regulation of genes involved in fish reproduction such as Vtg. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
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