Journal
VACCINE
Volume 22, Issue 13-14, Pages 1709-1716Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2003.09.051
Keywords
MIDGE; DNA vaccines; expression vectors
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The low efficacy obtained in large animals makes plasmid-based DNA vaccines commercially unviable. Another concern is the presence of antibiotic resistance markers on virtually all conventional plasmids. Here we describe the use of minimalistic, immunogenically defined gene expression (MIDGE) vectors for DNA vaccination. MIDGE are linear, covalently-closed vectors containing all the essential information for gene expression and none of the non-essential and potentially dangerous plasmid backbone sequences. MIDGE vectors can also be chemically modified on both ends at defined positions allowing targeting of the DNA to specific cell types or cellular compartments. Immunisation of mice with simple and end-modified MIDGE vectors showed that they are efficacious tools to generate and/or manipulate antigen-specific immune responses. (C) 2004 Elsevier Ltd. All rights reserved.
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