Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 101, Issue 16, Pages 5904-5909Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0305411101
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- NIGMS NIH HHS [R01 GM066380, GM-066380] Funding Source: Medline
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Recent studies from a number of laboratories have revealed a surprising number of connections between RNA polymerase 11 transcription and the ubiquitin/proteasome pathway. We now find yet another intersection of these pathways by showing that the 26S proteasome associates with regions of the GAL1, GAL10, and HSP82 genes, including the 3' ends, in a transcription-dependent fashion. The appearance of the proteasome on these inducible genes correlates with both the accumulation of transcripts and the buildup of RNA polymerase 11 complexes in the same region. Furthermore, the 26S proteasome and RNA polymerase 11 coimmunoprecipitate, and inhibition of 26S proteolytic activity leads to increased read through of a transcription termination site. We suggest that the proteasome is generally recruited to the DNA at sites of stalled RNA polymerase and may act to resolve these complexes.
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