Journal
JOURNAL OF CONTROLLED RELEASE
Volume 96, Issue 2, Pages 227-232Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jconrel.2004.01.024
Keywords
transfection microarray; human mesenchymal stem cells; fibronectin
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The transfection efficiency of primary cells is the bottleneck for their use with miniaturized formats for gene validation assays. We have found that when formulations containing various reporter plasmids were microarrayed on glass slides (chips), hMSCs cultivated on the chip incorporated and expressed the microarrayed plasmid DNAs with high efficiency and virtually total spatial resolution. Fibronectin, as the key formulation component, was found to significantly increase the on-chip transfection efficiency in hMSCs as well as many other cells. Further, we have conclusively proven that when siRNA was co-arrayed with the target plasmid DNA, a concentration-dependent gene knockdown was observed. Thus, massively miniaturized RNAi gene knockdown experiments can now be performed in primary cells, previously unusable with transfection microarrays (TMA). (C) 2004 Elsevier B.V. All rights reserved.
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