Journal
JOURNAL OF MOLECULAR BIOLOGY
Volume 338, Issue 3, Pages 439-444Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2004.02.070
Keywords
ribosome; translocation; tRNA; wobble; decoding
Categories
Funding
- NCI NIH HHS [CA09523-18] Funding Source: Medline
- NIGMS NIH HHS [R01-GM-23027-20] Funding Source: Medline
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Ribosomes translate genetic information encoded by messenger RNAs (mRNAs) into proteins. Accurate decoding by the ribosome depends on the proper interaction between the mRNA codon and the anticodon of transfer RNA (tRNA). tRNAs from all kingdoms of life are enzymatically modified at distinct sites, particularly in and near the anticodon. Yet, the role of these naturally occurring tRNA modifications in translation is not fully understood. Here we show that modified nucleosides at the first, or wobble, position of the anticodon and 3'-adjacent to the anticodon are important for translocation of tRNA from the ribosome's aminoacyl site (A site) to the peptidyl site (P site). Thus, naturally occurring modifications in tRNA contribute functional groups and conformational dynamics that are critical for accurate decoding of mRNA and for translocation to the P site during protein synthesis. (C) 2004 Elsevier Ltd. All rights reserved.
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