Journal
CANCER LETTERS
Volume 207, Issue 2, Pages 139-147Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2003.11.002
Keywords
E-NPP1; E-NPP3; bile duct carcinoma; primary biliary cirrhosis; carcinogenesis
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Ecto-nucleoticle phyrophosphatase/phosphodiesterase-1 enzyme (E-NPP) consists of three closely related molecules: E-NPP1, E-NPP2 and E-NPP3. We investigated the expression and localization of E-NPP1 and -3 in human inflammatory and neoplastic bile duct diseases. Immunohistochemically E-NPP I was located on the apical cytoplasmic side of cancer cells, whereas E-NPP3 was located in the apical plasma membrane. Western blot analysis revealed that the expression of E-NPP3, but not E-NPP1, was higher in tumor tissues than in surrounding tissues and the specific form of the E-NPP3 protein was readily detected in the sera of bile duct carcinoma (BDC) patients. Furthermore, it was confirmed that E-NPP3 was associated with migration ability by using of NIH3T3 cells that stably transfected with E-NPP3 cDNA. These results suggest that E-NPP3 is involved in the infiltration of neoplastic BDC and is possible to be a tumor marker. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
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