Journal
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
Volume 31, Issue 4, Pages 177-182Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-004-0134-9
Keywords
Geotrichum-like R59; medium optimization; thermostable lipase
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Growth and production of lipase by a new Geotrichum-like strain, R59, were studied. Production of extracellular lipase was substantially enhanced when the initial pH of the culture medium, types of carbon and nitrogen sources, substances probably stimulating the lipase biosynthesis, the temperature, and time of growth were optimized. Sucrose and triolein were the most effective carbon sources for lipase production. Maximum lipase activity (146 U/ml(-1)) was obtained with urea as the nitrogen source. Growth at 30degreesC, an initial pH of 6.0 and incubation time of 48 h were found as optimum conditions for cell growth and production of lipase by Geotrichum-like strain R59. The enzyme was thermostable and exhibited very high activity after I h incubation at 60degreesC.
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