Antibacterial effects of water-soluble low-molecular-weight chitosans on different microorganisms

Low-molecular-weight chitosans with a viscosity-average molecular weight (M-v) of 5 to 27 kDa and an equal degree of deacetylation (DD, 85%) were highly active against Pseudomonas aureqfaciens, Enterobacter agglomerans, Bacillus subtilis, and Bifidobacterium bifidum 791, causing death in 80 to 100% of cells. An exception to this tendency was Escherichia coli, for which the rate of cell death induced by the 5-kDa chitosan, was 38%. The antibacterial effect was manifested as early as 10 min after the incubation of 12-kDa chitosan with B. subtilis or E. coli cells. Candida krusei was almost insensitive to the above crab chitosans. However, Candida krusei was highly sensitive to chitosans with Mv 5, 6, 12, 15.7, and 27 kDa: the minimum inhibitory concentration (MIC) varied from 0.06 to 0.005%. Chitosans with M-v 5, 12, and 15.7 kDa exerted an antibacterial effect on Stapkylococcus aureus. Chitosans with M-v 5, 15.7, and 27 kDa had no effect on Bifidobacterium bifidum ATCC 14893. The antibacterial effect of the 4-kDa chitosan on E. coli and B. bifidum 791 increased with DID in the range 55-85%.