Morphological changes contribute to apoptotic cell death and are affected by caspase-3 and caspase-6 inhibitors during red sea bream iridovirus permissive replication

Red sea bream iridovirus (RSIV) of the Iridoviridae family is a causative agent of lethal infections in many cultured marine fish species in southwestern Japan. RSIV-induced apoptosis was divided as follows: (1) cell shrinkage and rounding at the early apoptotic stage, (2) cell enlargement at the middle apoptotic stage, (3) formation of apoptotic body-like vesicles at the late apoptotic stage and phagocytosis by neighboring cells, and (4) loss of membrane integrity in apoptotic body-like vesicles without phagocytosis by neighboring cells. By affinity labeling, RSIV-induced apoptosis included caspase-dependent apoptosis. RSIV infection caused cell rounding but not cell enlargement or formation of apoptotic body-like vesicles and further restricted part of the structural protein synthesis in the presence of caspase-3 and -6 inhibitors. These findings showed the involvement of caspase-3 and -6 in the morphological changes at the middle and late apoptotic stages and viral protein synthesis in the late stage of RSIV infection. (C) 2004 Elsevier Inc. All rights reserved.