Journal
ENVIRONMENTAL POLLUTION
Volume 129, Issue 2, Pages 237-245Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.envpol.2003.10.025
Keywords
Ca-dependent Cd-tolerance; transgenic; tobacco; LCT1
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Wheat cDNA LCT1, a nonspecific transporter for Ca2+, Cd2+, Na+ and K+, was overexpressed in tobacco. Transformants were tested for their sensitivity to a range of Ca2+-concentrations [0.01-10 mM Ca(NO3)(2)] with or without the presence of 0.05 mM Cd(NO3)(2). Calcium and cadmium accumulation was also determined. LCT1-transformed plants expressed a phenotype distinct from controls only under conditions of low calcium (0.01-1 mM Ca2+). They grew significantly better and had slightly higher shoot calcium concentration. Transformants subjected to 0.05 mM Cd(NO3)(2) in the presence of I mM Ca2+ displayed a substantially higher level of tolerance to cadmium and accumulated less Cd in roots. These results are the first to demonstrate the involvement of LCT1 in calcium acquisition and in the regulation of amelioration of Cd-toxicity by calcium. (C) 2003 Elsevier Ltd. All rights reserved.
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