Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 57, Issue 2, Pages 279-281Publisher
ELSEVIER
DOI: 10.1016/j.mimet.2003.12.006
Keywords
DGGE; double bands; artifacts
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Denantrant gradient gel electrophoresis (DGGE) is a widely used method for mutation analysis and for studies of microbial diversity. Particular combinations of target gene fragments and primers may give rise to erroneous DGGE profiles. We report on a very straightforward means to eliminate the artifactual 'double bands' that can be encountered in several applications. (C) 2004 Elsevier B.V All rights reserved.
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