Journal
NUCLEIC ACIDS RESEARCH
Volume 32, Issue 9, Pages 2873-2879Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkh613
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Funding
- NHGRI NIH HHS [HG01642] Funding Source: Medline
- NIGMS NIH HHS [T32 GM007616, 5T32-GM07616] Funding Source: Medline
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We have developed the first fully integrated microfluidic system for DNA sequencing-by-synthesis. Using this chip and fluorescence detection, we have reliably sequenced up to 4 consecutive bps. The described sequencer can be integrated with other microfluidic components on the same chip to produce true lab-on-a-chip technology. The surface chemistry that was designed to anchor the DNA to elastomeric microchannels is useful in a broad range of studies and applications.
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