Journal
AQUATIC TOXICOLOGY
Volume 132, Issue -, Pages 182-189Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aquatox.2013.02.013
Keywords
Tigriopus japonicus; Copper; Cytotoxicity; Apoptosis; Oxidative stress; TUNEL assay; BrdU assay; p38 MAPK
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Funding
- MOMAF [201200000002729]
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The copepod, Tigriopus japonicus is an important model for toxicity testing. However, no attempt has been made in analyzing the effect of toxicants at the level of the ROS-mediated signal transduction pathway. To understand copper-induced cytotoxicity at the molecular level, we employed several cellular and biochemical assays after exposure to copper, and found a significant induction of enzyme activities of antioxidant proteins with increased intracellular reactive oxygen species (ROS) as well as an increase of TUNEL-positive cells, but a decrease of BrdU-positive cells. In addition, several important genes such as p38 MAPK, antioxidant-related genes, Hsps, and apoptosis-related genes were significantly modulated by copper exposure. Taken together, we suggest that copper-induced cytotoxicity is mediated by the formation of intracellular ROS and oxidative stress in T. japonicus. Whole body biochemical assays such as TUNEL- and BrdU-assay will provide a better understanding of cellular responses such as apoptosis and cell death upon cytotoxic exposure of copper in T. japonicus. (C) 2013 Elsevier B.V. All rights reserved.
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