Journal
MICROBIAL PATHOGENESIS
Volume 36, Issue 5, Pages 287-292Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2004.01.001
Keywords
Burkholderia pseudomallei; Burkholderia thailandensis; plaque formation; adherence; invasion; human respiratory epithelium
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Burkholderia pseudomallei, a causative agent of melioidosis, is a facultative intracellular gram-negative bacillus that is closely related to its avirulent counterpart, Burkholderia thailandensis. However, pathogenic mechanisms and virulence factors of B. pseudomallei remain elusive. In the present study, we compared the invasiveness, adherence, and replication of B. pseudomallei and B. thailandensis in human respiratory epithelial cells A549. Invasion was determined after 4 h of coculturing using antibiotic protection assay. Adherence was demonstrated by coculturing the cells with fluorescein-labeled bacteria for I h and the number of positive cells was analyzed by flow cytometry. The results obtained with this in vitro study demonstrated that compared with its avirulent counterpart, B. pseudomallei is significantly more efficient (P < 0.01) in invasion, adherence and inducing cellular damage, as represented by plaque formation. (C) 2004 Elsevier Ltd. All rights reserved.
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