Supercritical carbon dioxide extraction of 2-acetyl-1-pyrroline and volatile components from panclan leaves

The flavour of pandan (Pandanus amaryllifolius Roxb.) leaves was extracted by supercritical fluid with Col (SC-CO2) under different conditions of pressure, temperature and contact time to determine the yield of 2-acetyl-1-pyrroline (ACPY) and various other components; 14 volatile compounds on the gas chromatogram, were identified, and the predominant constituents were ACPY and 3-methyl-2(5H)-furanone. The interaction of different conditions significantly influenced the yield of ACPY and various volatile compounds. There is a potential for high yield of ACPY by SC-CO2 at 200 bar, 500 degreesC and 20 min. The SDE-ether extract was found to have a very small amount of ACPY and an undesirable odour, as compared to the dark green ethanol extract, which contains a relatively larger quantity of ACPY and even more 3-methyl-2(5H)-furanone. Although at least 34 new components were uncovered from SC-CO2, SIDE, and ethanol extraction, both ACPY and 3-methyl-2(5H)-furanone were the components tentatively obtained by all three methods. Copyright (C) 2004 John Wiley Sons, Ltd.