Journal
JOURNAL OF MOLECULAR RECOGNITION
Volume 17, Issue 3, Pages 194-197Publisher
WILEY
DOI: 10.1002/jmr.664
Keywords
protein detection; affinity probes; proximity ligation; padlock probes; genotyping; gene expression; rolling circle replication
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Procedures and reagents are needed to specifically detect all the macromolecules that are being identified in the course of genome projects. We discuss how this challenge may be met using a set of ligation-based reagents termed padlock probes and proximity ligation probes. These probes include elements with affinity for specific nucleic acid and protein molecules, respectively, along with unique identifier DNA sequence elements that encode the identity of the recognized target molecules. The information content of DNA strands that form in the detection reactions are recorded after amplification, allowing the recognized target molecules to be identified. The procedures permit highly specific solution-phase or localized analyses of large sets of target molecules as required in future molecular analyses. Copyright (C) 2004 John Wiley Sons, Ltd.
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