Journal
EMBO REPORTS
Volume 5, Issue 5, Pages 497-502Publisher
WILEY
DOI: 10.1038/sj.embor.7400128
Keywords
chromatin assembly; homologous recombination; sister chromatid exchange; DNA replication
Categories
Ask authors/readers for more resources
Histone chaperone Asf1 participates in heterochromatin silencing, DNA repair and regulation of gene expression, and promotes the assembly of DNA into chromatin in vitro. To determine the influence of Asf1 on genetic stability, we have analysed the effect of asf1Delta on homologous recombination. in accordance with a defect in nucleosome assembly, asf1Delta leads to a loss of negative supercoiling in plasmids. importantly, asf1Delta increases spontaneous recombination between inverted DNA sequences. This increase correlates with an accumulation of double-strand breaks (DSBs) as determined by immunodetection of phosphorylated histone H2A and fluorescent detection of Rad52-YFP foci during S and G2/M phases. In addition, asf1Delta shows high levels of sister chromatid exchange (SCE) and is proficient in DSB-induced SCE as determined by physical analysis. Our results suggest that defective chromatin assembly caused by asf1Delta leads to DSBs that can be repaired by SCE, affecting genetic stability.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available