Journal
JOURNAL OF MICROSCOPY-OXFORD
Volume 214, Issue -, Pages 174-181Publisher
BLACKWELL PUBLISHING LTD
DOI: 10.1111/j.0022-2720.2004.01347.x
Keywords
autofluorescence; confocal microscopy; multiphoton microscopy; spectral imaging; unmixing
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Fluorescence imaging at high spectral resolution is now a practical reality and has great promise in plant cell biology. Emission spectral curve data can be used computationally to distinguish spectrally similar fluorophores, or to remove autofluorescence, and to spectrally analyse autofluorescent molecules, which are especially abundant in plant tissues. Examples of these applications in plant cells are given, and a comparison is made between the current offerings in spectral imaging laser scanning confocal microscopes.
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