Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 126, Issue 17, Pages 5446-5451Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja035550m
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- NIGMS NIH HHS [GM62958-01] Funding Source: Medline
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Fluorescence spectra show that excitation of the cationic water-soluble conjugated polymer poly-{ (1,4-phenylene)-2,7-[9,9-bis(6'-N,NN-trimethylammonium)-hexyl]fluorene diiodide} (1) results in inefficient fluorescence resonance energy transfer (FRET) to ethidium bromide (EB) intercalated within double-stranded DNA (dsDNA). When fluorescein (Fl) is attached to one terminus of the dsDNA, there is efficient FRET from 1 through Fl to EB. The cascading energy-transfer process was examined mechanistically via fluorescence decay kinetics and fluorescence anisotropy measurements. These experiments show that the proximity and conformational freedom of Fl provide a FRET gate to dyes intercalated within DNA which are optically amplified by the properties of the conjugated polymer. The overall process provides a substantial improvement over previous homogeneous conjugated polymer based DNA sensors, namely, in the form of improved selectivity.
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