Journal
VACCINE
Volume 22, Issue 15-16, Pages 1921-1929Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2003.11.015
Keywords
European Bat Lyssavirus 1; hemi-nested reverse transcriptase polymerase chain reaction; reference rabies diagnostic methods
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A simplified hemi-nested reverse transcriptase polymerase chain reaction (hnRT-PCR) has been developed to determine specifically the European Bat Lyssavirus 1 (EBLV-1) nucleoprotein gene. The specificity of this method was determined by using the seven genotypes of lyssavirus by RT-PCR, Southern blot and sequence analysis. Compared to the rabies diagnostic methods, the hnRT-PCR showed a higher sensitivity for the detection of small amounts of EBLV-1 virus. In view of these results, we suggest this new hnRT-PCR should be performed for the epidemiological survey of bat colonies, also providing rapid detection and genotyping of EBLV-1 until now encountered in all naturally infected bats in France. (C) 2003 Elsevier Ltd. All rights reserved.
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